How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

Unlike DNA, which is mainly double stranded, RNA is double stranded, although an RNA molecule can self-hybridize at certain regions to form partial double-stranded structures.

A

True

B

False

Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________

A

polishing and end filling respectively

B

end filling and polishing respectively

C

polishing in both the cases

D

end filling in both the cases

Consider the following statements: 1. DNA contains the sugar deoxyribose, while RNA contains the sugar ribose. 2. DNA is a double-stranded molecule, while RNA is a single-stranded molecule. 3. DNA is not stable under alkaline conditions, while RNA is stable. Which of the given above statements is/are correct?

A

1 and 2

B

2 only

C

2 and 3

D

1, 2 and 3

Consider the following statements : 1. Adenoviruses have single-stranded DNA genomes whereas retroviruses have double-stranded DNA genomes. 2. Common cold is sometime caused by an adenovirus whereas AIDS is caused by a retrovirus. Which of the statements given above is/are correct?

A

1 only

B

2 only

C

Both 1 and 2

D

Neither 1 nor 2

If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification?

A

10,420

B

5,120

C

20,480

D

10,240

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

A

1, 2, 3 and 4 are true

B

Only 2 and 4 are true

C

Oniy 2, 3 and 4 are true

D

Only 1 and 2 are true

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs⁺ DNA is given by the equation ρ = 1.66 + 0.098 X_{G + C}, where X_{G + C} denotes

A

Total number of G and C

B

Mole fraction of G + C

C

Number of GC repeats

D

Ratio of G + C to A + T content

Which of the following statements are correct? 1. The DNA Technology (Use and Application) Regulation Bill, 2019 provides for the regulation of use of DNA technology for establishing the identity of certain persons. 2. DNA is made up of 26 pairs of chromosomes and provides instructions for building an entire organism and proteins. 3. DNA is composed of two chains, which coil around each other to form a double helix carrying the genetic instructions used in the growth. 4. Under the Bill, DNA profiling is allowed only in respect of criminal matters. Select the correct answer using the code given below:

A

Only 1 and 2

B

Only 2 and 3

C

Only 1 and 3

D

1, 2, 3 and 4

Single stranded form combines cloning, amplification and __________________ of and originally double-stranded DNA fragment.

A

Strand separation

B

Replication

C

Transcription

D

Translation

Which of the following statements about DNA is/are correct?
1. DNA is the hereditary material of all living organisms.
2. All segments of DNA code for synthesis of proteins.
3. Nuclear DNA is double helical with two nucleotide chains which run anti-parallel.
4. DNA is also found in mitochondria.
Select the correct answer:

A

1, 2 and 3

B

3 and 4

C

1, 3 and 4

D

Only 4

How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

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