Single stranded form combines cloning, amplification and __________________ of and originally double-stranded DNA fragment.

Single stranded form combines cloning, amplification and __________________ of and originally double-stranded DNA fragment. Correct Answer Strand separation

Single-stranded form is an attractive means of combining the cloning, amplification and strand separation of an originally double-stranded DNA fragment.
Bissoy MCQ

Related Questions

If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification?
Unlike DNA, which is mainly double stranded, RNA is double stranded, although an RNA molecule can self-hybridize at certain regions to form partial double-stranded structures.
Which one of the combinations of the following statements is true about antibody structure?
P. Limited proteolysis of rabbit IgG with the enzyme pepsin generates two antigen-binding regions (Fab) and an Fc fragment.
Q. Limited proteolysis of rabbit IgG with the enzyme papain generates a single bivalent antigen-binding region F(ab')2 and peptide fragments.
R. The Fc fragment of IgG can self-associate and crystallize into a lattice.
S. The F(ab')2 fragment of IgG is composed of both light and heavy chains.
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Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is
Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
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Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor
When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________
You take a crude cell lysate and treat it with DNase. Now wash it and add protease. Use specific restriction endonuclease to get your desired fragment and run it on a gel. You see a single band. Which of the following will be true about the fragment?