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Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Which among the following statements regarding filing Rejoinder/Replication in a suit, as per the Code of Civil Procedure 1908, are correct?
1. Rejoinder/ Replication is filed as a matter of Right
2. Provision of filing Rejoinder/ Replication is contained under Order VIII of the Code of Civil Procedure
3. Rejoinder/Replication may be filed only after due permission of the Court.
4. Rejoinder/Replication is a part of pleading.
Select the correct answer:
In an experiment you use DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.
If colEI derived origin of replication is replaced by the origin of replication of F plasmid, it is called as:
Column A lists the cell parts and Column B lists the functions of the cell part. Column A Column B i. Cell wall A. Control what happens ii. Cell membrane B. Supports the cell. iii. Cytoplasm C. Controls what passes in and out of the cell. iv. Nucleus D. Cell reactions happen in it. Which one of the following options is correct?
Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is
If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?
When electroporation is used for introducing DNA into mammalian cell
P. A carrier for DNA is not required.
Q. The lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites.
R. The viability of the cell becomes approximately zero percent.
S. The first step involves absorption of DNA on the cell membrane.
Consider the following statements regarding cell cycle and choose the correct answer:
1. During G1 phase the cell grows in size and there is an active synthesis of RNA and proteins
2. The third stage of the cell cycle is termed G2 phase
3. In the mitotic phase, the DNA molecule of each chromosome replicates by the synthesis of a new DNA molecule on the template of the existing DNA
Replication can also be carried out by rolling circle replication. Choose the correct statement for this type of replication?