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If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?

If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe? Correct Answer At first small fragment, then two separate bands showing long fragment with increasing length and short fragments of definite length.

The small fragments are the Okazaki fragments of lagging strand while the longer is the leading stand DNA. While leading strand grows in length the Okazaki fragments in absence of ligation to each other results in small fragments of definite length.

Related Questions

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.
Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Which among the following statements regarding filing Rejoinder/Replication in a suit, as per the Code of Civil Procedure 1908, are correct?
1. Rejoinder/ Replication is filed as a matter of Right
2. Provision of filing Rejoinder/ Replication is contained under Order VIII of the Code of Civil Procedure
3. Rejoinder/Replication may be filed only after due permission of the Court.
4. Rejoinder/Replication is a part of pleading.
Select the correct answer:
Directions : In each of the following questions a statement is given, followed by two conclusions. Give answer :

Statement : The General Administration Department has issued a circular to all the employees informing them that henceforth the employees can avail their lunch break at any of the half-hour slots between 1.00 p.m. and 2.30 p.m.

Assumptions :
I. The employees may welcome the decision and avail lunch break at different time's slots.
II. There may not be any break in the work of the organization as the employees will have their lunch break at different time slots.
Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?
Consider the following statements about the genetic materials DNA and RNA: DNA chemically is less reactive and structurally more stable as compared to RNA. DNA is better for the transmission of genetic information as compared to RNA. Both DNA and RNA can mutate. Which of the statements given above are correct?
The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase
What is the reaction in DNA replication catalyzed by DNA ligase?
In an experiment you use DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.
In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?