Replication can also be carried out by rolling circle replication. Choose the correct statement for this type of replication?
Replication can also be carried out by rolling circle replication. Choose the correct statement for this type of replication? Correct Answer It can be used for the formation of RF
Rolling circle replication is used for the formation of RF. The product of gene II binds on a specific site of double stranded genome. It creates a nick in the + strand generating a free 3’ hydroxyl. This strand is extended by the polymerase and it displaces the original + strand. This strand is removed from the newly synthesized strand because of another nick by gene II. Thus a separate + strand is created and it is circularized by gene II product.
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Feb 20, 2025