Which of the following enzyme is assayed and measured using spectrofluorometric method?
Which of the following enzyme is assayed and measured using spectrofluorometric method? Correct Answer Hydrolases
Hydrolases can be assayed by measuring the rate of appearance of fluorescence at 450nm of the anion 4-methylumbelliferone. Phosphofructokinase is assayed and measured using spectrophotometer. Hydrolases is measured by using spectrofluorometric method, whereas bacterial luciferase is measured by using luminescence method. Glucose-6-phosphate dehydrogenase can be assayed by using continuous assay.
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Feb 20, 2025