Which of the following is not true for spectrofluorometric method?
Which of the following is not true for spectrofluorometric method? Correct Answer The intensity of emitted light is used to study enzyme reaction
“The intensity of emitted light is used to study enzyme reaction.” This is true of luminescence method and not spectrofluorometric method. Following statements are true for spectrofluorometric method are as follows: • Trace impurities in enzyme can stop the emitted radiation. • It is based on stoke shift. The increase in wavelength due to absorption in UV region and emission in visible light is known as stoke shift. • Hydrolases can be assayed and measured by using this method.
মোঃ আরিফুল ইসলাম
Feb 20, 2025