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In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?

In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography? Correct Answer Southern blotting

Southern blotting is the technique where nucleic acids are first transferred from gel to a nitrocellulose membrane. The membrane is then incubated in a solution of probes (complementary sequences) and then the bound sequences analyzed using autoradiography.

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Determine the correctness of the following statements.
I. Enhancer sequences are those DNA sequences that are involved in increasing the rate of DNA replication.
II. Enhancer sequences work by binding with eukaryotic gene activator factors.
A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?
Consider the following statements about DNA Sequencing: 1. DNA Sequencing is used to detect the presence of a complementary sequence by hybridization with a nucleic acid sample. 2. DNA sequencing is the process of determining the nucleic acid sequence - the order of nucleotides in DNA. Which of the above statements is/are incorrect?
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
Which of the following statements are correct? 1. The DNA Technology (Use and Application) Regulation Bill, 2019 provides for the regulation of use of DNA technology for establishing the identity of certain persons. 2. DNA is made up of 26 pairs of chromosomes and provides instructions for building an entire organism and proteins. 3. DNA is composed of two chains, which coil around each other to form a double helix carrying the genetic instructions used in the growth. 4. Under the Bill, DNA profiling is allowed only in respect of criminal matters. Select the correct answer using the code given below:
Regarding DNA fingerprinting, consider the following statements: It is a technique to find out variations in individuals of a population at the DNA level. It works on the principle of polymorphism in DNA sequences. DNA fingerprinting can be used for paternity testing. Which of the statements given above is/are correct?
_______ analyzed the distribution of scores among 100 vertebrate nucleic acid sequences and compared these scores with randomized sequences prepared in different ways.
Which of the following statements about DNA is/are correct? 1) DNA is the hereditary material of all living organisms. 2) All segments of DNA code for the synthesis of proteins. 3) Nuclear DNA is double helical with two nucleotide chains that run anti-parallel. 4) DNA is also found in mitochondria. Select the correct answer using the code given below:
The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.