Nucleic-acid Techniques MCQ

Test your knowledge with important Nucleic-acid Techniques MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Nucleic-acid Techniques MCQs at Bissoy

• Which of the following is a commonly used label in blotting techniques?
  ক

  vimentin

  খ

  biotin

  গ

  avidin

  ঘ

  streptomycin
• Which of the following molecules can be analyzed using a northern blot?
  ক

  RNA

  খ

  Carbohydrates

  গ

  Proteins

  ঘ

  DNA
• In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?
  ক

  Western blotting

  খ

  Southern blotting

  গ

  Isopycnic centrifugation

  ঘ

  Spectrophotometry
• The procedure by which DNA gel is transferred to a membrane is termed as ___________________
  ক

  blotting

  খ

  centifugation

  গ

  electrophoresis

  ঘ

  paper chromatography
• Nucleic acid hybridization is used to identify ________________________
  ক

  RNAs

  খ

  DNAs

  গ

  Complementary base sequences

  ঘ

  Proteins
• Which technique can be used to separate 15N and 14N?
  ক

  Rate-zonal centrifugation

  খ

  Isopycnic centrifugation

  গ

  HPLC

  ঘ

  Mass spectrometry
• Equilibrium centrifugation is also known as ___________________ centrifugation.
  ক

  isotonic

  খ

  isopycnic

  গ

  gradient

  ঘ

  sucrose
• In rate-zonal sedimentation, the nucleic acids are separated base on __________________
  ক

  nucleotide length

  খ

  relative charge

  গ

  relative mass

  ঘ

  mass-to-charge ratio
• One Svedberg unit is equivalent to 10-13 ______________
  ক

  hours

  খ

  minutes

  গ

  seconds

  ঘ

  milli-seconds
• Which technique is used to separate nucleic acids of size greater than 25 kb?
  ক

  SDS-PAGE

  খ

  Pulsed-field electrophoresis

  গ

  2D- gel electrophoresis

  ঘ

  Isoelectric focusing
• Which type of gel is used for large nucleic acids?
  ক

  acrylamide

  খ

  cellulose

  গ

  agarose

  ঘ

  sephadex
• Phenol is an active protein denaturant.
  ক

  True

  খ

  False
• Which of the following is used to precipitate the nucleic acids?
  ক

  glycerol

  খ

  ethanol

  গ

  methanol

  ঘ

  ethylene
• In purification steps of nucleic-acids, phenol is used for _______________________
  ক

  deproteinization

  খ

  denaturation

  গ

  lowering pH

  ঘ

  lowering viscosity
• Which of the following is used to lyse the nucleus and release the DNA?
  ক

  sodium dodecyl sulfate

  খ

  ammonium sulfate

  গ

  ferric phosphate

  ঘ

  fluorine