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The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.

The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template. Correct Answer True

For accurate work, a printout of the scan is usually examined for abnormalities that decrease the quality of the sequence, and the sequence may then be edited manually. The first sequence can also be extended by making a second oligonucleotide matching the distal end of the readable sequence and using this primer to read more of the original template.

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________
Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease
Who were the first to suggest that one strand of DNA might act as a template for the synthesis of its complementary strand?
Word 2010 has a particular template that it uses when you first open the program. This template contains default styles, no text and you also use it when you create new, blank documents. As you customize styles, marcos and other elements these settings are automatically stored in this template. What is the name of this template file?
Watson and Crick’s suggestion of the complementary strand synthesis taking one of the parent strand as template was proposed in _________
In eukaryotes, which DNA polymerase is involved in extending the RNA primer and providing template for an accessory factor and replication factor, which control the processivity in lagging strand?
The primer used for lagging strand synthesis in prokaryotes is an RNA primer.
A : T ratio in one strand of DNA duplex is 2, what will be the ratio in the complementary strand
Which of the following enzymes catalyses the synthesis of RNA molecules complementary to single-strand DNA template?