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Which set of the following events occurs during the elongation step of translation?
P. Attachment of mRNA with the smaller subunit of ribosome
Q. Loading of correct aminoacyl-tRNA into the A site.
R. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P site.
S. Dissociation of the ribosomal subunits.
T. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome.
Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.
If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.
Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.
Which of the following statements about gene therapy are correct?
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be
Which ribosome recognized the transcription initiation site of the mRNA?
If give a segment – 5’CTGACCAUGGCCTAGAUGCCTCT, which AUG will the eukaryotic ribosome choose as initiation site?
Given below are six sentences, four of which (P,Q,R,S) are jumbled. Pick the option that gives the correct order of P,Q,R,S. 1 The site proposed at Miyapur is located close to the main road. P - The title deed of the land is clear and no litigation is envisaged. Q - Further, as there is a shopping complex close to the site, water and electricity may not be a problem. R - Firstly, it is easily accessible as it well connected to various parts of the city by three bus routes. S - Most important of all, the building adjacent to the site is also available on lease. 6 There is scope for expansion of the office as a plot of land on the north of the proposed site is a vacant at present.
Which of the following statements is/are correct? 1. Gene Editing deals with inserting genes from other organism while Gene Modification deals with altering genes that exists already within body. 2. Gene Modification process is time consuming while Gene editing is faster. Select the correct answer using the code given below.