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Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.

Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. Correct Answer True

Expression of cloned sequences in E. coli requires a promoter, a ribosome binding site and an initiation codon. Initiation codon is the start codon AUG.

Related Questions

If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.
Which set of the following events occurs during the elongation step of translation?
P. Attachment of mRNA with the smaller subunit of ribosome
Q. Loading of correct aminoacyl-tRNA into the A site.
R. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P site.
S. Dissociation of the ribosomal subunits.
T. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome.
The initiation codon of the gene is located ___________ of the ribosome binding site.
TSSW is a web program that distinguishes promoter sequences from non-promoter sequences based on a combination of unique content information such as hexamer/trimer frequencies and signal information such the TATA box in the promoter region.
In EM algorithm, as an example, suppose that there are 10 DNA sequences having very little similarity with each other, each about 100 nucleotides long and thought to contain a binding site near the middle 20 residues, based on biochemical and genetic evidence. the following steps would be used by the EM algorithm to find the most probable location of the binding sites in each of the ______ sequences.
Eukaryotic RNA polymerase II does not directly bind to the promoter, but relies on a dozen or more transcription factors to recognize and bind to the promoter in a specific order before its own binding around the promoter.
Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.
Determine the correctness of the following statements.
I. Enhancer sequences are those DNA sequences that are involved in increasing the rate of DNA replication.
II. Enhancer sequences work by binding with eukaryotic gene activator factors.
You are the administrator of a Windows 2000 Server computer named Intra. Intra is a member of an Active Directory domain and hosts an Intranet Web-Ske for your company. Company policy requires that only authenticated users have access to the intranet site. All company users have a user account in the Active Directory domain. You configure directory security for the Web Site to use integrated security. However, you discover that users can access the Web Site without authentication. You need to ensure that only authenticated users can access the web site. What should you do?
Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency.