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Mismatched DNA does not affect the rate of activity of DNA polymerase.

Mismatched DNA does not affect the rate of activity of DNA polymerase. Correct Answer False

Te accuracy of the base – pairing is monitored by the palm domain of the polymerase. The palm makes extensive hydrogen bond contacts with the base pairs in the minor groove of the newly synthesized strand. These contacts are not base pair specific but only bond if the base – pairing is correct. Thus mismatched DNA dramatically slows the rate of activity of DNA polymerase.

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg2+ concentration
Q. pH
R. Annealing temperature
Which of the following statements are true/false?
1. A minimum rate of wages for the time spent by employees for the production of output is minimum time rate.
2. Minimum piece rate is the rate based on the quantity of the output employees have produced.
3. The guaranteed time rate is a minimum rate of remuneration to apply in the case of employees employed on piece work for the purpose of securing to such employees a minimum rate of wages on a time work basis.
4. A minimum rate of remuneration to apply in the case of employees employed on piece work for the purpose of securing to such employees a minimum rate of wages on a time work basis is overtime rate.
The Taq DNA polymerase is DNA polymerase _________ enzyme from the bacterium Thermus aquaticus.
Which of the following characteristics with respect to bacterial DNA polymerase III are true?
P. Initiation of chain synthesis
Q. 5' → 3' polymerization
R. 3' → 5' exonuclease activity
S. 5' → 3' exonuclease activity
You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?
S1: Different countries show different pattern of growth.
P: Many others have a high birth rate with a low death rate.
Q: Some have a high birth rate and still have a high death rate.
R: The developing countries show the most rapid growth rate.
S: Some others like the European nations, have a low birth rate and a low death rate.
S6: Compared to this in Europe the growth rate is low.

The Proper sequence should be:
DNA polymerase II can polymerase upto ______________ nucleotides per minute at 37˚C.
A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg2+. Which one of the following is missing in the reaction mixture?