During electrophoresis denaturation of the double stranded DNA is brought about by __________

During electrophoresis denaturation of the double stranded DNA is brought about by __________ Correct Answer Treatment with alkali

During electrophoresis denaturation of the double stranded DNA is brought about by its treatment with alkali. This step is done once the sample is separated by electrophoresis and taken up in the nitrocellulose membrane.
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In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?