Hybridization of DNA and RNA MCQ

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• The process by which a probe is used to screen a library is known as ___________
  ক

  Hybridization

  খ

  Southern blotting

  গ

  Colony hybridization

  ঘ

  Western blotting
• Northern blotting is performed for ____________
  ক

  Determining the size of DNA

  খ

  Determining the size of RNA

  গ

  Quantification of RNA

  ঘ

  Sequencing of RNA
• In case of northern blotting the hybridization occurs between two RNA molecules.
  ক

  True

  খ

  False
• During electrophoresis denaturation of the double stranded DNA is brought about by __________
  ক

  Treatment with alkali

  খ

  Application of current

  গ

  Treatment with EtBr

  ঘ

  Application of heat
• Radiolabelling is generally brought about by addition of radioactive phosphorus.
  ক

  True

  খ

  False
• Fluorescent labeling is an important phenomenon in case of hybridization and manipulations in molecular biology.
  ক

  True

  খ

  False
• What is the function of polynucleotide kinase?
  ক

  Addition of γ – phosphate at 3’ – OH

  খ

  Addition of γ – phosphate at 5’ – OH

  গ

  Removal of γ – phosphate at 3’ – OH

  ঘ

  Removal of γ – phosphate at 5’ – OH
• In how many ways a DNA can be labeled?
  ক

  2

  খ

  3

  গ

  4

  ঘ

  5
• Why is a probe labeled?
  ক

  Improve visibility

  খ

  Improve stability

  গ

  Improve location identification

  ঘ

  Improve binding capability
• What is a probe?
  ক

  Chemically synthesized DNA

  খ

  Purified DNA

  গ

  Fragmented DNA duplex

  ঘ

  Either purified or synthesized single stranded DNA
• Which of the following is not termed as hybridization?
  ক

  DNA and cDNA

  খ

  DNA and mRNA

  গ

  DNA from different species

  ঘ

  DNA from male and female of same species
• Which of the following does not affect the formation of hybrid DNA?
  ক

  Ionic strength

  খ

  Pressure

  গ

  Temperature

  ঘ

  Homologous DNA