In how many ways a DNA can be labeled?

A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?

A

4 labeled; 6 unlabeled

B

4 labeled; 4 unlabeled

C

3 labeled; 5 unlabeled

D

3 labeled; 3 unlabeled

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

A

1, 2, 3 and 4 are true

B

Only 2 and 4 are true

C

Oniy 2, 3 and 4 are true

D

Only 1 and 2 are true

Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because

A

The sequence context at the RB defines the direction of transfer

B

The sequence context at the LB defines the direction of transfer

C

The nuclear location sequence (NLS) of VirD2 protein drives the excised T-strand

D

The endonuclease activity of VirD2 protein allows nicking at RB

Which of the following statements about DNA is/are correct?
1. DNA is the hereditary material of all living organisms.
2. All segments of DNA code for synthesis of proteins.
3. Nuclear DNA is double helical with two nucleotide chains which run anti-parallel.
4. DNA is also found in mitochondria.
Select the correct answer:

A

1, 2 and 3

B

3 and 4

C

1, 3 and 4

D

Only 4

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

A

3, 1, 2, 4, 6, 5, 7, 8

B

1, 4, 3, 2, 5, 7, 6, 8

C

1, 2, 6, 7, 5, 8, 3, 4

D

8, 6, 7, 5, 1 , 4, 2, 3

Given below are two statements, one labeled as Assertion (A) and the other labeled as Reason (R). Read the statements and choose the correct answer:
Assertion (A): Proclamation of emergency can be issued by the President of India if there is an imminent danger of war or external aggression or internal disturbance and armed rebellion.
Reason (R): The President of India can suspend the enforcement of some fundamental rights during the period of emergency.

A

Both A and R are true and R is the correct explanation of A

B

Both A and R are true, but R is not the correct explanation of A

C

A is true, but R is false

D

A is false, but R is true

Consider the following statements regarding the DNA-mediated gene transfer and choose the correct answer:
1. DNA fragments carrying the gene to be transferred are mixed with a carrier DNA
2. They are then precipitated out with a calcium phosphate buffer
3. The precipitate is used to transform target cells
4. The Ca-precipitated DNA enters the cell by exocytosis

A

Only 1, 2 and 4 are true

B

Only 3 and 4 are true

C

Only 2, 3 and 4 life true

D

Only 1, 2 and 3 are true

Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease

A

1, 2, 3 and 4 are true

B

Only 2, 3 and 4 are true

C

Only 1, 2 and 3 are true

D

Only 1, 3 and 4 are true

Consider the following statements regarding satellite DNA and choose the correct answer:
1. Satellite or Repetitive DNA consists of short identical genes which are repeated in tandem several hundred or thousand times
2. Such DNA is found in the region of the chromosome adjacent to the centromere
3. It is easy to separate repetitive DNA by ultracentrifugation

A

Only 1 and 2 are true

B

Only 2 and 3 are true

C

Only 1 and 3 are true

D

1, 2 and 3 are true

Batch of cells labeled as A: In the hypoxic condition, the amount of dosage required is 3 Gy but in oxygenic condition, the amount of dosage needed is 2. Batch of cells labeled as B: In hypoxic condition, the amount of dosage required is 30 Gy but in oxygenic conditions, the amount of dosage needed is 10. From the above information, what conclusions can be drawn?

A

Batch A is not cancerous

B

Batch B has a higher OER

C

Batch A has a higher OER

D

Batch B is cancerous

In how many ways a DNA can be labeled?

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