How do we measure the concentration of the enzyme?
How do we measure the concentration of the enzyme? Correct Answer In units of activity
The mass of a particular enzyme added to a reaction is rarely known; most commercial enzyme preparations contain several components in unknown and variable proportions depending on the batch obtained from the manufacturer. To overcome these difficulties, enzyme quantity is often expressed as units of activity measured under specified conditions. One unit of enzyme is usually taken as the amount which catalyses conversion of 1μmol substrate per minute at the optimal temperature, pH and substrate concentration.
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Feb 20, 2025