Immobilised lactase is used to hydrolyse lactose in dairy waste to glucose and galactose. Enzyme is immobilised in resin particles and packed into a 0.5 m3 column. The total effectiveness factor for the system is close to unity; Km for the immobilised enzyme is 1.32 kg m-3; Vmax is 45 kg m-3 h-1. The lactose concentration in the feed stream is 9.5 kg m-3; a substrate conversion of 98% is required. The column is operated with plug flow for a total of 310 d per year. At what flow rate should the reactor be operated?
Immobilised lactase is used to hydrolyse lactose in dairy waste to glucose and galactose. Enzyme is immobilised in resin particles and packed into a 0.5 m3 column. The total effectiveness factor for the system is close to unity; Km for the immobilised enzyme is 1.32 kg m-3; Vmax is 45 kg m-3 h-1. The lactose concentration in the feed stream is 9.5 kg m-3; a substrate conversion of 98% is required. The column is operated with plug flow for a total of 310 d per year. At what flow rate should the reactor be operated? Correct Answer 1.56 m3 h-1
For 98% substrate conversion, sf = (0.02 sI) = 0.19 kg m-3
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Feb 20, 2025