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What will be the consequence of not having an origin of replication (ori) in the vector?

What will be the consequence of not having an origin of replication (ori) in the vector? Correct Answer If an ori is absent, replication of vector would not take place

In the case ori is absent, the vector won’t be able to replicate. As the replication won’t take place, only one of the daughter cells would be having the vector. A colony of transformed colonies won’t be obtained. The puc18 ori came from a plasmid in clinical bacterial isolate and was called as pMB1.

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