Bissoy.com
Doctors Medicines MCQs Q&A

Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?

Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning? Correct Answer The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector

In the case of this type of cloning, the restriction site is induced into the PCR primers and the enzyme is induced into the vector. The enzyme cuts the PCR product at the restriction site and joins it to the vector. Topoisomerase I is responsible for cutting only one strand and Topoisomerase II cuts both the strands.

Related Questions

Simplify the value of $$\frac{{{\text{0}}{\text{.9}} \times {\text{0}}{\text{.9}} \times {\text{0}}{\text{.9 + 0}}{\text{.2}} \times {\text{0}}{\text{.2}} \times {\text{0}}{\text{.2 + 0}}{\text{.3}} \times {\text{0}}{\text{.3}} \times {\text{0}}{\text{.3}} - {\text{3}} \times 0.9 \times {\text{0}}{\text{.2}} \times {\text{0}}{\text{.3}}}}{{{\text{0}}{\text{.9}} \times {\text{0}}{\text{.9 + 0}}{\text{.2}} \times {\text{0}}{\text{.2 + 0}}{\text{.3}} \times {\text{0}}{\text{.3}} - 0.9 \times {\text{0}}{\text{.2}} - {\text{0}}{\text{.2}} \times {\text{0}}{\text{.3}} - 0.3 \times 0.9}} = ?$$
$$\frac{{38 \times 38 \times 38 + 34 \times 34 \times 34 + 28 \times 28 \times 28 - 38 \times 34 \times 84}}{{38 \times 38 + 34 \times 34 + 28 \times 28 - 38 \times 34 - 34 \times 28 - 38 \times 28}}$$            is equal to = ?
Which of the following statement (s) is/are true about the following eliminations? (1) Hoffmann product is major product in I (2) Saytzeff product is major product in I (3) Hoffmann product is major product in II (4) Saytzeffproduct is major product in II
$$\eqalign{ & {\text{The}}\,{\text{value}}\,{\text{of}} \cr & \frac{{0.1 \times 0.1 \times 0.1 + 0.02 \times 0.02 \times 0.02}}{{0.2 \times 0.2 \times 0.2 + 0.04 \times 0.04 \times 0.04}}\,\text{is}: \cr} $$
The value of $$\left( {\frac{{0.1 \times 0.1 \times 0.1 + 0.02 \times 0.02 \times 0.02}}{{0.2 \times 0.2 \times 0.2 + 0.04 \times 0.04 \times 0.04}}} \right)$$       is :
The value of $${\text{ }}\root 3 \of {\frac{{0.2 \times 0.2 \times 0.2 + 0.04 \times 0.04 \times 0.04}}{{0.4 \times 0.4 \times 0.4 + 0.08 \times 0.08 \times 0.08}}} $$        is ?
Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________
Emulsion or droplet PCR is another modification of PCR. Which of the statement holds true?
TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?
In this question a passage and a statement related to it have been given. Read carefully the passage and review the statement based on it. When Shah Jahan gave his love for Mumtaz, he would have believed that the river would continue to flow. There was no solid land on which the foundation could be laid. Therefore, to reduce the level of water, he used an engineering system to use the foundation of deep well and it was filled with stone and mud. Finally, he covered them with wooden box work structure and the tomb Made Yamuna is an integral part of the creation of the Taj and there was no such predict ion that it would dry up or become narrow. But the river was narrow and it became contaminated. The timber of wood is likely to be brittle or disintegrated as the venetian made on the foundation of wood, indicates that when the wood is submerged in the wood, the age of the wood increases. When the wood does not come in contact with oxygen, then that But such creatures cannot be found who destroy them. Due to the dryness and contamination of the Yamuna, the breakdown of a wooden tree can break. Statement. To maintain the structure of the TajMahal, a revival of Yamuna river is necessary. Choose one of the following options. A. The statement is definitely true. B. The statement is probably true. C. Statement cannot be determined D. Statement is definitely wrong.