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In the case of blunt-end ligation, blunt ends can be generated by ___________

In the case of blunt-end ligation, blunt ends can be generated by ___________ Correct Answer both the action of restriction endonuclease which gives straight ends and polishing of staggered ends

Blunt ends are those which don’t have single stranded ends as overhangs. The ends are double stranded. And these can be generated by polishing the staggered ends or the action of restriction endonuclease which generate straight ends.

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Blunt ends created by the restriction endonuclease can be joined.
How many categories of ligation reaction are there on the basis of ends created?
Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor
Which of the following is not a method for putting sticky ends to a blunt ended DNA fragment to be cloned?