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In enzymology, diguanylate cyclase, also known as diguanylate kinase , is an enzyme that catalyzes the chemical reaction:

2 Guanosine triphosphate ↔ 2 diphosphate + cyclic di-3',5'-guanylate

The substrates of diguanylate cyclases are two molecules of guanosine triphosphate and the products are two molecules of diphosphate and one molecule of cyclic di-3’,5’-guanylate.

Degradation of cyclic di-GMP to guanosine monophosphate is catalyzed by a phosphodiesterase.

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