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Doctors Medicines MCQs Q&A

  1. Protein
  2. Bile salt
  3. Glucose
  4. Ketone
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1 Answers

Option 3 : Glucose

Concept:-

  • Benedict's test: A test for detecting glucose in urine in which urine is heated with a blue solution containing sodium carbonate, sodium citrate, and copper sulfate, producing a red, yellow, or orange precipitate in the presence of glucose.

Procedure:

  • take 5 ml of benedict’s reagent.
  • Add 8 drops of carbohydrate solution.
  • Boil over a flame or in a boiling water bath for 2 minutes
  • Let the solution cool.

Principle:

  • Carbohydrates with a free aldehyde or ketone groups have the ability to reduce solutions of various metallic ions.
  • Reducing sugars under alkaline conditions tautomerize and form enediols.
  • Enediols are powerful reducing agents.
  • They reduce cupric ions to cuprous form and are themselves converted to sugar acids.
  • The cuprous ions combine with OH- ions to form yellow cuprous hydroxide which upon heating is converted to red cuprous oxide.  

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