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Enzyme responsible for nick translation on the lagging strand, during DNA replication, is :

Enzyme responsible for nick translation on the lagging strand, during DNA replication, is : Correct Answer DNA polymerase I

Concept -

  • DNA replication is a semi-conservative process in which two parental strands separate and each acts as a template for synthesizing a complementary strand over it. 
  • After completion of replication, each DNA had one parental strand and one newly synthesized strand of DNA.
  • Replication of DNA occurs in the S phase of the cell cycle.
  • During the S phase, the amount of DNA present In each cell doubles.
  • If the initial amount of DNA is denoted as 2C then it increases to 4C.

Explanation-

  • The formation of new chains is catalyzed by an enzyme DNA Polymerase.
  • It is of three types- DNA polymerase I, DNA polymerase II and, DNA Polymerase III.
  • DNA Polymerase I was discovered by KORNBERG.
  • This enzyme functions as an exonuclease.
  • It separates RNA primer from DNA and also fills the gap.
  • It is also known as DNA- repair enzyme.

Therefore the enzyme responsible for nick translation on the lagging strand, during DNA replication, is DNA Polymerase I.

Additional Information

DNA Polymerase III

  • This is the main enzyme for replication.
  • The larger chains are formed by this enzyme.
  • It was discovered by Delucia and Cairns.

Topoisomerase

  • Topoisomerase is an enzyme that removes torsion.

Related Questions

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1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
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