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Doctors Medicines MCQs Q&A

Identify the correct statements.
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction.
Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid.
R. T4 polynucleotide kinase is used for labeling 3' end of DNA.
S. Baculovirus (Autographa californica) can be used as an insect expression vector.

Identify the correct statements.
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction.
Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid.
R. T4 polynucleotide kinase is used for labeling 3' end of DNA.
S. Baculovirus (Autographa californica) can be used as an insect expression vector. Correct Answer P and S only

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease
You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________
The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase
Autographa californica nuclear polyhedrois virus (AcNPV) is commonly used bacilovirus for infecting cultured cells. The virus is ________ and _______
AP endonuclease can cleave the DNA at__________ while AP lyase will cleave the DNA at__________
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
A signaling cascade is illustrated by protein kinase protein kinase protein kinase and MAP. What purpose does this cascade serve?
A linear Hamming code is used to map 4-bit messages to 7-bit codewords. The encoder mapping is linear. If the message 0001 is mapped to the codeword 0000111, and the message 0011 is mapped to the codeword 1100110, then the message 0010 is mapped to
Consider a system described by ẋ = Ax + Bu y = Cx + Du The system is completely output controllable if and only if Where: x = State vector (n-vector) u = Control vector (r-vector) y = Output vector (m-vector) A = n × n matrix B = n × r matrix C = m × n matrix D = m × r matrix