A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?

A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication? Correct Answer Southern blotting

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Duplication of Vermillion eye colour gene leads to the expression of the mutant phenotype. Which of the following can lead to normal phenotype with this duplication present?