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A bacterial culture having a specific oxygen uptake rate of 5 mmol O2 (g DCW)-1 hr-1 is being grown aerobically in a fed-batch bioreactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s-1 for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg mL-1). The maximum density to which the cells can be grown in the fed-batch process without the growth being limited by oxygen transfer, is approximately

A bacterial culture having a specific oxygen uptake rate of 5 mmol O2 (g DCW)-1 hr-1 is being grown aerobically in a fed-batch bioreactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s-1 for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg mL-1). The maximum density to which the cells can be grown in the fed-batch process without the growth being limited by oxygen transfer, is approximately Correct Answer 65 g L<sup>-1</sup>

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In a well aerated and agitated microbial culture, the 'supply' of oxygen is equal to 'demand' (uptake) of the growing culture. The KLa for such a system will be (KLa = volumetric mass transfer coefficient, C* = dissolved oxygen concentration in liquid in equilibrium with gaseous oxygen, C = instantaneous value of dissolved oxygen concentration, 'r' = specific oxygen uptake rate per unit weight of cells, X = dry weight of the cells per unit volume)
Three pipes x, y and z can fill a tank A can fill it in 4 minutes. Two pipes p and q are connected to tank B. how much time does it take for both tanks to run at the full capacity? Ratio of time taken to fill tank A by P and time taken by x to fill tank A is 2: 1 and ratio of time taken by Q to fill tank B and Z to fill tank A is 5: 3. Pipe P is 20% more efficient then pipe Q. and pipe Y takes 12 minutes to fill the tank. Capacity of tank B is 3/4 times of tank A. Note: Tank B is to be filled only after filling tank A. Valves of pipe p and q are closed once tank B is running at full capacity.
In the following question, two statements are numbered as A and B. On solving these statements, we get quantities A and B respectively. Solve both quantities and choose the correct option. Quantity A: Pipe A takes thrice the time taken by Pipe B to fill a tank. Together they can fill the tank in 15 hours, tank A and B is opened together for 4 hours and then Pipe A is closed and after 2 more hours Pipe B is closed and an outlet Pipe C is opened which when working alone can empty the tank in 10 hours. Calculate the time taken by C to empty the tank. Quantity B: A pipe A can fill the tank in 10 hours another pipe B can fill the tank in 15 hours and with the help of third pipe C they can fill the tank in 5 hours. Pipe A is opened for 4 hours then it is closed, pipe B is closed 3 hours before filling of tank and tank C is opened the whole time. Calculate the time taken to fill the tank
In the following question, two statements are numbered as A and B. On solving these statements, we get quantities A and B respectively. Solve both quantities and choose the correct option. Quantity A: Pipe A takes thrice the time taken by Pipe B to fill a tank. Together they can fill the tank in 15 hours, tank A and B is opened together for 4 hours and then Pipe A is closed and after 2 more hours Pipe B is closed and an outlet Pipe C is opened which when working alone can empty the tank in 10 hours. Calculate the time taken by C to empty the tank. Quantity B: A pipe A can fill the tank in 10 hours another pipe B can fill the tank in 15 hours and with the help of third pipe C they can fill the tank in 5 hours. Pipe A is opened for 4 hours then it is closed, pipe B is closed 3 hours before filling of tank and tank C is opened the whole time. Calculate the time taken to fill the tank.
A strain of Azotobacter vinelandii is cultured in a 15 m3 stirred fermenter for alginate production. Under current operating conditions kLa is 0.17 s-1. Oxygen solubility in the broth is approximately 8 x 10-3 kg m-3. The specific rate of oxygen uptake is 12.5 mmol g-1 h-1. What is the maximum possible cell concentration?
A bacterial culture with an appropriate biomass composition of CH1.8O0.5N0.2 is grown aerobically on a defined medium containing glucose as the sole carbon source and ammonia being the nitrogen source. In this fermentation, biomass is formed with a yield coefficient of 0.35 gram dry cell weight per gram of glucose and acetate is produced with a yield coefficient of 0.1 gram acetate per gram of glucose. The respiratory coefficient for the above culture will be
A tank has an optional adjustable outlet pipe that can be attached to the tank at any height we want. This outlet pipe drains water from the tank at a constant rate, while the tank is being filled using another inlet pipe at a constant rate. If the outlet, pipe is attached at half the height of the tank, the tank, which is initially empty gets filled in 18 hours. If the outlet pipe is attached at height equal to two third height of the tank, the tank, which is initially empty, is filled in 16 hours. In how many hours will the tank, which is initially empty, be completely filled if the outlet pipe is not attached to the tank?
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Wash out (as defined by D = μmax) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S0 = substrate concentration in feed, P = product concentration in bioreactor).
A 18-year-old patient presented with hypertension and recurrent weakness of all four limbs. His serum Na+ 140 mmol/L, serum K+ 2.6 mmol/L, serum HC03" 30 mmol/L. Probable cause/s is/are