Bioprocess Engineering And Process Biotechnology MCQ

Test your knowledge with important Bioprocess Engineering And Process Biotechnology MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Bioprocess Engineering And Process Biotechnology MCQs at Bissoy

• Match the entries in Group I with the process parameters in Group II.<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Group I</strong></td> <td><strong>Group II</strong></td> </tr> <tr> <td>P. Clark electrode</td> <td>1. Liquid level</td> </tr> <tr> <td>Q. Redox probe</td> <td>2. Dissolved oxygen concentration</td> </tr> <tr> <td>R. Load cell</td> <td>3. Vessel pressure</td> </tr> <tr> <td>S. Diaphragm gauge</td> <td>4. pH (anaerobic process)</td> </tr></table> P-2, Q-1, R-3, S-4

  খ

  P-4, Q-2, R-3, S-1

  গ

  P-2, Q-4, R-1, S-3

  ঘ

  P-2, Q-1, R-4, S-3
• Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm^-1. At a concentration of 50 mg L^-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm^-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L^-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
  ক

  21.4 mg cm^-3

  খ

  214 mg cm^-3

  গ

  2.14 mg cm^-3

  ঘ

  None of the above
• In large-scale fermentation, the preferred method of sterilization is
  ক

  Chemicals

  খ

  Radiation

  গ

  Filtration

  ঘ

  Heat
• In large scale fermentation process, air is sterilized by
  ক

  Jute fiber

  খ

  Membrane

  গ

  Cotton fiber

  ঘ

  Glass wool fiber
• If the dissociation constant for solute-adsorbent binding is K_D, the retention time of the solute in a chromatography column
  ক

  Increases with increasing K_D

  খ

  Decreases with increasing K_D

  গ

  Passes through minimum with increasing K_D

  ঘ

  Is independent of K_D
• An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than one could be apparently due to one of the following reasons. Identify the correct reason.
  ক

  The enzyme follows substrate inhibited kinetics with internal pore diffusion limitation

  খ

  The enzymes experiences external film diffusion limitation

  গ

  The enzyme follows sigmoidal kinetics

  ঘ

  The immobilized enzyme is operationally unstable
• Determine the correctness or otherwise of the following Assertion (A) and Reason (R).<br>Assertion: Bacterial growth is called synchronous when majority of the cells are in same stage of the bacterial cell cycle.<br>Reason: Synchronous culture can be obtained by growing bacteria in an enriched medium
  ক

  Both (A) and (R) are true and (R) is the correct reason for (A)

  খ

  Both (A) and (R) are true but (R) is not the correct reason for (A)

  গ

  (A) is true but (R) is false

  ঘ

  (A) is false but (R) is true
• Which of the following techniques is not ideal for immobilizing cell-free enzymes?
  ক

  Physical entrapment by encapsulation

  খ

  Covalent chemical bonding to surface carriers

  গ

  Physical bonding by flocculation

  ঘ

  Covalent chemical bonding by cross-linking the precipitate
• The specific growth rate (μ) of a microorganism in death phase is
  ক

  0 (zero)

  খ

  μ_max

  গ

  Less than zero

  ঘ

  Greater than zero
• Aeration in a bioreactor is provided by
  ক

  Impeller

  খ

  Baffles

  গ

  Sparger

  ঘ

  All of the above
• Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?
  ক

  Denaturing polyacrylamide gel electrophoresis

  খ

  Size-exclusion chromatography

  গ

  Ion-exchange chromatography

  ঘ

  Nickel affinity chromatography
• Diauxic pattern of biomass growth is associated with<br>P. Multiple lag phases.<br>Q. Sequential utilization of multiple substrates.<br>R. Simultaneous utilization of multiple substrates.<br>S. Absence of lag phase.
  ক

  P, R

  খ

  P, Q

  গ

  R, S

  ঘ

  Q, S
• If the fractional recovery at each step of unit operation is 0.8, the recovery after 4 steps will be
  ক

  0.24

  খ

  3.23

  গ

  0.41

  ঘ

  0.82
• The limiting factor in the production of large quantities of ethanol as biofuel is
  ক

  Lack of a balanced medium

  খ

  Ethanol toxicity to cells

  গ

  Expensive downstream processing steps

  ঘ

  Only B and C of the above
• A bacterial culture having a specific oxygen uptake rate of 5 mmol O₂ (g DCW)^-1 hr^-1 is being grown aerobically in a fed-batch bioreactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s^-1 for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg mL^-1). The maximum density to which the cells can be grown in the fed-batch process without the growth being limited by oxygen transfer, is approximately
  ক

  14 g L^-1

  খ

  26 g L^-1

  গ

  32 g L^-1

  ঘ

  65 g L^-1
• Immobilization of enzymes<br>P. Increases the specificity of the enzyme for its reactants.<br>Q. Facilitates reuse of the enzyme in batch reactions.<br>R. Makes it unsuitable for its use in a continuous reactor system.<br>S. Decreases the operational cost of the industrial process.
  ক

  Q, S

  খ

  Q, R

  গ

  R, S

  ঘ

  P, Q
• Match the bioreactor components in Group I with the most appropriate function given in Group II.<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Group I</strong></td> <td><strong>Group II</strong></td> </tr> <tr> <td>P. Marine type impeller</td> <td>1. Recirculation of medium</td> </tr> <tr> <td>Q. Draft tube</td> <td>2. Aeration of medium</td> </tr> <tr> <td>R. Diaphragm valve</td> <td>3. Animal cell cultivation</td> </tr> <tr> <td>S. Sparger</td> <td>4. Sterile operation</td> </tr></table> P-4, Q-2, R-1, S-3

  খ

  P-3, Q-1, R-4, S-2

  গ

  P-3, Q-4, R-2, S-1

  ঘ

  P-2, Q-1, R-4, S-3
• How many kilograms of ethanol is produced from 1 kilogram of glucose in ethanol fermentation?
  ক

  2.00

  খ

  0.20

  গ

  0.51

  ঘ

  0.05
• A bioreactor is scaled up based on equal impeller tip speed. Consider the following parameters for small and large bioreactors:<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Parameters</strong></td> <td><strong>Small bioreactor</strong></td> <td><strong>Large bioreactor</strong></td> </tr> <tr> <td>Impeller speed</td> <td>N₁</td> <td>N₂</td> </tr> <tr> <td>Diameter of impeller</td> <td>D₁</td> <td>D₂</td> </tr> <tr> <td>Power consumption</td> <td>P₁</td> <td>P₂</td> </tr></table><br>Assuming geometrical similarity and the bioreactors are operated in turbulent regime, what will be $$\frac{{{P_2}}}{{{P_1}}}$$ ? $${\left( {\frac{{{D_1}}}{{{D_2}}}} \right)^2}$$

  খ

  $${\left( {\frac{{{D_2}}}{{{D_1}}}} \right)^2}$$

  গ

  $${\left( {\frac{{{D_1}}}{{{D_2}}}} \right)^5}$$

  ঘ

  $${\left( {\frac{{{D_2}}}{{{D_1}}}} \right)^5}$$
• A culture vessel in which physical, physicochemical and physiological conditions, as well as cell concentration, are kept constant is known as
  ক

  Cell concentrator

  খ

  Biostat

  গ

  Batch bioreactor

  ঘ

  Incubator
• Cibacron Blue dye affinity chromatography can be used for affinity purification of
  ক

  NADPH dehydrogenase

  খ

  Glucoamylase

  গ

  Subtilisin

  ঘ

  Caspase
• Substrate consumption in lag phase of microbial growth is primarily used for<br>P. Turnover of the cell material.<br>Q. Maintenance of intracellular pH.<br>R. Motility.<br>S. Increase in cell number.
  ক

  P, Q and S only

  খ

  Q, R and S only

  গ

  P, Q and R only

  ঘ

  S only
• In chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than maximum growth rate by
  ক

  Partial cell recycling

  খ

  Using sub-optimal temperature

  গ

  pH cycling

  ঘ

  Substrate feed rate cycling
• Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase?
  ক

  μ = 0

  খ

  μ

  গ

  μ = μ_max

  ঘ

  μ max
• Which of the following statements is incorrect about immobilized plant cell cultures?
  ক

  It is possible to use high cell densities

  খ

  Cells remain active for long periods

  গ

  Cell products or inhibitors can be removed easily

  ঘ

  It provides low shear resistance to cells
• To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation?
  ক

  Culture agitation to maintain oxygen supply

  খ

  Restriction of the entry of contaminating organisms

  গ

  Control of parameters like pH and temperature

  ঘ

  Maintenance of constant culture volume
• Match each parameter in Group I with the appropriate measuring device in Group II.<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Group I</strong></td> <td><strong>Group II</strong></td> </tr> <tr> <td>P. Pressure</td> <td>1. Photometer</td> </tr> <tr> <td>Q. Foam</td> <td>2. Rotameter</td> </tr> <tr> <td>R. Turbidity</td> <td>3. Diaphragm gauge</td> </tr> <tr> <td>S. Flow rate</td> <td>4. Rubber sheathed electrode</td> </tr></table> P-3, Q-4, R-1, S-2

  খ

  P-1, Q-3, R-2, S-4

  গ

  P-4, Q-1, R-2, S-3

  ঘ

  P-1, Q-2, R-3, S-4
• Wash out (as defined by D = μ_max) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S₀ = substrate concentration in feed, P = product concentration in bioreactor).
  ক

  X = 0, S = 0, P = 0

  খ

  X = 0, S = S₀, P = 0

  গ

  X = 0, S

  ঘ

  X
• Which of the following statements applies to the operation of a fed-batch process?
  ক

  The volume of the culture increases with time

  খ

  It helps controlling repressive effects of the nutrient being fed

  গ

  It eliminates the need for oxygen supply

  ঘ

  Only A and B of the above
• Which of the following statements is always correct about an ideal chemostat?<br>P. Substrate concentration inside the chemostat is equal to that in the exit stream.<br>Q. Optimal dilution rate is lower than critical dilution rate.<br>R. Biomass concentration increases with increase in dilution rate.<br>S. Cell recirculation facilitates operation beyond critical dilution rate.
  ক

  P and Q only

  খ

  P, R and S only

  গ

  P and S only

  ঘ

  P, Q and S only