Bioprocess Engineering And Process Biotechnology MCQ
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Match the entries in Group I with the process parameters in Group II.<br>
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Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm<sup>-1</sup>. At a concentration of 50 mg L<sup>-1</sup> of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm<sup>-1</sup>. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L<sup>-1</sup> protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
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In large-scale fermentation, the preferred method of sterilization is
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In large scale fermentation process, air is sterilized by
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If the dissociation constant for solute-adsorbent binding is K<sub>D</sub>, the retention time of the solute in a chromatography column
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An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than one could be apparently due to one of the following reasons. Identify the correct reason.
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Determine the correctness or otherwise of the following Assertion (A) and Reason (R).<br>Assertion: Bacterial growth is called synchronous when majority of the cells are in same stage of the bacterial cell cycle.<br>Reason: Synchronous culture can be obtained by growing bacteria in an enriched medium
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Which of the following techniques is not ideal for immobilizing cell-free enzymes?
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The specific growth rate (μ) of a microorganism in death phase is
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Aeration in a bioreactor is provided by
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Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?
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Diauxic pattern of biomass growth is associated with<br>P. Multiple lag phases.<br>Q. Sequential utilization of multiple substrates.<br>R. Simultaneous utilization of multiple substrates.<br>S. Absence of lag phase.
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If the fractional recovery at each step of unit operation is 0.8, the recovery after 4 steps will be
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The limiting factor in the production of large quantities of ethanol as biofuel is
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A bacterial culture having a specific oxygen uptake rate of 5 mmol O<sub>2</sub> (g DCW)<sup>-1</sup> hr<sup>-1</sup> is being grown aerobically in a fed-batch bioreactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s<sup>-1</sup> for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg mL<sup>-1</sup>). The maximum density to which the cells can be grown in the fed-batch process without the growth being limited by oxygen transfer, is approximately
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Immobilization of enzymes<br>P. Increases the specificity of the enzyme for its reactants.<br>Q. Facilitates reuse of the enzyme in batch reactions.<br>R. Makes it unsuitable for its use in a continuous reactor system.<br>S. Decreases the operational cost of the industrial process.
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Match the bioreactor components in Group I with the most appropriate function given in Group II.<br>
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How many kilograms of ethanol is produced from 1 kilogram of glucose in ethanol fermentation?
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A bioreactor is scaled up based on equal impeller tip speed. Consider the following parameters for small and large bioreactors:<br>
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A culture vessel in which physical, physicochemical and physiological conditions, as well as cell concentration, are kept constant is known as
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Cibacron Blue dye affinity chromatography can be used for affinity purification of
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Substrate consumption in lag phase of microbial growth is primarily used for<br>P. Turnover of the cell material.<br>Q. Maintenance of intracellular pH.<br>R. Motility.<br>S. Increase in cell number.
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In chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than maximum growth rate by
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Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase?
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Which of the following statements is incorrect about immobilized plant cell cultures?
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To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation?
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Match each parameter in Group I with the appropriate measuring device in Group II.<br>
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Wash out (as defined by D = μ<sub>max</sub>) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S<sub>0</sub> = substrate concentration in feed, P = product concentration in bioreactor).
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Which of the following statements applies to the operation of a fed-batch process?
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Which of the following statements is always correct about an ideal chemostat?<br>P. Substrate concentration inside the chemostat is equal to that in the exit stream.<br>Q. Optimal dilution rate is lower than critical dilution rate.<br>R. Biomass concentration increases with increase in dilution rate.<br>S. Cell recirculation facilitates operation beyond critical dilution rate.