Which statement correctly describes sequential steps in cDNA cloning?

Which statement correctly describes sequential steps in cDNA cloning? Correct Answer Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector

Bissoy MCQ

Related Questions

cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).
cDNA made from the mRNA of an organism was used to make a cDNA library in a vector that allows the expression as a fusion with a reporter tag. What percentage of the cDNA clones is likely to give rise to correct gene products?
Three cats are roaming in a zoo n such a way that when cat A takes 5 steps, B takes 6 steps and C takes 7 steps.But the 6 steps of A are equal to the 7 steps of B and 8 steps of C. what is the ratio of their speeds?
In order to isolate cDNA for a specified protein from a mice cDNA library prepared in λgt11 vector which of the following methods you will choose for screening?
A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?
Mr. Ali decided to walk down the escalator of a tube station. He found that if he walks down 26 steps, he requires 30 seconds to reach the bottom. However, if he steps down 34 stairs he would only require 18 seconds to get to the bottom. If the time is measured from the moment the top step begins to descend to the time he steps off the last step at the bottom, find out the height of the stair way in steps?
A policeman starts to chase a thief. When the thief goes 10 steps the policeman moves 8 steps and 5 steps of the policeman are equal to 7 steps of the thief. The ratio of the speed of the policeman and the thief is = ?
Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?
Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?
TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?