Methods And Techniques In Applied Biology MCQ

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• Which statement correctly describes sequential steps in cDNA cloning?
  ক

  Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector

  খ

  mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector

  গ

  mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector

  ঘ

  Double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector
• Which of the following is a semi-continuous fermentation in which only a small amount of substrate is available at the beginning of the process?
  ক

  Feed-back fermentation

  খ

  Fed-batch fermentation

  গ

  Reduced Fermentation

  ঘ

  None of the above
• Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?
  ক

  A missense mutation within the protein coding region of a gene

  খ

  A silent mutation within the protein coding region of a gene

  গ

  A single base change within the intron sequence of a gene

  ঘ

  An error in RNA splicing that mistakenly removes an exon during RNA processing
• Which of the following is a desirable characteristic for a cloning plasmid?
  ক

  A site at which replication can be initiated

  খ

  One or more unique restriction endonuclease sites

  গ

  One or more antibiotic-resistance or drug resistance genes

  ঘ

  All of the above
• Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately
  ক

  4 : 8

  খ

  8 : 4

  গ

  1 : 64

  ঘ

  1 : 26
• Genetically engineered male sterile crop plants may be produced by inserting
  ক

  BT toxin gene

  খ

  Barnase gene

  গ

  Lectin gene

  ঘ

  Chitinase gene
• ELISA is used to detect viruses where the key reagent is
  ক

  Alkaline phosphatase

  খ

  Catalase

  গ

  DNA probe

  ঘ

  Rnase
• Restriction endonucleases hydrolyzes polynucleotide from
  ক

  Only the 5' end

  খ

  From either terminal

  গ

  At an internal phosphodiester bond

  ঘ

  A phosphodiester bond within a specific sequence
• Which of the following are not valid methods of labelling duplex DNA?
  ক

  5'-end labelling with polynucleotide kinase

  খ

  3'-end labelling with polynucleotide kinase

  গ

  3'-end labelling with terminal transferase

  ঘ

  Nick translation
• Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?
  ক

  In situ hybridization

  খ

  DNA microarray

  গ

  Protein microarray

  ঘ

  Reporter gene fusion including introns
• Match List-I (cloning vector) with List-II (character) and select the correct answer:<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>List-I: Cloning vector</strong></td> <td><strong>List-II: Character</strong></td> </tr> <tr> <td>a. Yeast episomal plasmid (YEp)</td> <td>1. Integrates with host chromosome for replication</td> </tr> <tr> <td>b. Yeast integrative plasmid (YIp)</td> <td>2. Carries a chromosomal origin of replication</td> </tr> <tr> <td>c. Yeast replicative plasmid (YRp)</td> <td>3. Carries origin of replication of the 2 mm plasmid</td> </tr></table> a-3, b-2, c-1

  খ

  a-2, b-1, c-3

  গ

  a-3, b-1, c-2

  ঘ

  a-2, b-3, c-1
• The genome sequencing of rice is important because
  ক

  The rice genome is very unique and contains genes not found in other plants

  খ

  The rice genome is very large compared to the DNA of other grains and so more genes will be identified

  গ

  It can identify genes associated with disease resistance, growth capacity, etc

  ঘ

  It has a rapid life cycle and has many identifiable mutations
• The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?
  ক

  EcoRI (G/AATTC)

  খ

  XmaIII (C/GGCGG)

  গ

  SmaI (CCC/GGG)

  ঘ

  SacII (CCGC/GG)
• If the linear double stranded genomic DNA of bacteriophage T4 is heated and cooled, one gets both linear and circular molecules because,
  ক

  All linear DNA on heating and cooling attain circular shape

  খ

  The DNA molecules are circularly permuted

  গ

  The DNA molecule has cohesive terminal ends

  ঘ

  The RNA component of the genomes gets hydrolyzed to results in the circularization
• Free amino acids can be determined with the help of
  ক

  Heterotrophic bacteria

  খ

  Auxotrophic bacteria

  গ

  Algae

  ঘ

  Fungi
• Which statement about restriction enzymes are false?
  ক

  Restriction enzymes cut DNA at specific sequence called recognition sites

  খ

  A restriction enzyme always cut DNA to leave the same sequence at the ends

  গ

  Some restriction enzymes cut the two DNA strands at slightly different points within their recognition site to make a 'sticky' end

  ঘ

  Restriction enzymes are exonucleases rather than endonucleases
• Hybridoma technology is used in
  ক

  Synthesis of haemoglobin

  খ

  Production of alcohol

  গ

  Production of somatic hybrids

  ঘ

  Synthesis of monoclonal antibodies
• The sensitivity of ELISA is
  ক

  10^-6 g/ml

  খ

  10^-7 g/ml

  গ

  10^-9 g/ml

  ঘ

  10^-12 g/ml
• RF =
  ক

  $$\frac{{{\text{Distance from last point to origin}}}}{{{\text{Distance from origin run by the solvent}}}}$$

  খ

  $$\frac{{{\text{Distance from origin run by the compound}}}}{{{\text{Distance from origin run by the solvent}}}}$$

  গ

  $$\frac{{{\text{Distance from origin run by the solvent}}}}{{{\text{Distance from origin run by the compound}}}}$$

  ঘ

  None of the above
• In Southern blotting, DNA is fixed on
  ক

  Nitrocellulose

  খ

  Nylon membrane

  গ

  A and B both

  ঘ

  None of these
• The technique which involves addition or deletions of genes is
  ক

  Gene therapy

  খ

  Genetic engineering

  গ

  Gene splicing

  ঘ

  Artificial synthesis
• The process used for separation of protein in polyacrylamide gel is called:
  ক

  Southern blotting

  খ

  Northern blotting

  গ

  Western blotting

  ঘ

  Eastern blotting
• For protoplast fusion to be successful in plant cells.
  ক

  Fusion agents other than polyethylene glycol should be used

  খ

  Cell wall of the two strains of cells should not be damaged

  গ

  DNA between the two cells should be compatible

  ঘ

  Osmolarity of the medium is not important
• The ability to clone an adult animal from the nucleus of a differentiated somatic cell proves that
  ক

  The germ line and soma are identical

  খ

  Genetic engineering is a powerful technique

  গ

  Animals are not all that different from plants

  ঘ

  The nuclei of somatic cells contain all the genetic information present in a fertilized egg
• Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?
  ক

  The products of the drug resistance genes protect the cDNA from destruction by the host cells

  খ

  The drug resistance genes provide additional base sequences that enable the vector to accommodate larger inserts of cDNA

  গ

  Entry of the vector containing the cDNA and the drug resistance genes into the host cell renders the latter identifiable as it is now resistant to antibiotic drugs

  ঘ

  The cloned cDNA imparts drug resistance upon any cellular system with which it is used
• Cells deficient in hypoxanthine guanine phospho-ribosyl transferase (HGPRT)
  ক

  Synthesis of purine deoxynucleotides by salvage pathway

  খ

  Synthesis of purine deoxynucleotides by de novo path way

  গ

  Supply of hypoxanthine in the culture medium

  ঘ

  Supply of thymidine in the culture medium enzyme rely on
• A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be
  ক

  1 Kbp

  খ

  &gt; 2 Kbp

  গ

  0.5 Kbp

  ঘ

  1.5 Kbp
• Northern blotting is developed by
  ক

  Ed Southern

  খ

  Thomas et al

  গ

  Alwine et al

  ঘ

  E. Northern
• A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is
  ক

  Phage mid

  খ

  Cosmid

  গ

  Plasmid

  ঘ

  YAC
• In agarose gel electrophoresis
  ক

  DNA migrates towards the negative electrode

  খ

  Supercoiled plasmids migrate slower than their nicked counterparts

  গ

  Larger molecules migrate faster than smaller molecules

  ঘ

  Ethidium bromide can be used to visualize the DNA