Restriction endonucleases which recognize and cut same recognition sequences are known as

Restriction endonucleases from two different organisms that recognize the same DNA sequence for cleavage are called

A

Isoschizomers

B

Isozymes

C

Concatemers

D

Palindromes

The restriction endonuclease Eco52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco52I?

A

EcoRI (G/AATTC)

B

XmaIII (C/GGCGG)

C

SmaII (CCCC/GGG)

D

SacII (CCGC/GG)

The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?

A

EcoRI (G/AATTC)

B

XmaIII (C/GGCGG)

C

SmaI (CCC/GGG)

D

SacII (CCGC/GG)

Assertion (A): Scientists can cut apart and paste together DNA molecules at will, regardless of the source of the molecules.
Reason (R): DNA fragments can be manipulated using restriction endonucleases and DNA ligases.

A

Both (A) and (R) are true and (R) is the correct explanation of (A)

B

Both (A) and (R) are true, but (R) is not the correct explanation of (A)

C

(A) is true, but (R) is false

D

(A) is false, but (R) is true

Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately

A

4 : 8

B

8 : 4

C

1 : 64

D

1 : 26

A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be

A

4096 bp

B

256 bp

C

16 bp

D

436 bp

Which of the following is true about restriction endonucleases?

A

Type I and II requires ATP to move along DNA

B

Type I, II and III requires ATP to move along DNA

C

Type II requires no ATP and cleaves DNA within recognition sequence

D

Type II requires ATP and cleaves DNA within recognition sequence

Which type of restriction endonucleases is used most in genetic engineering?

A

Type I

B

Type II

C

Type III

D

Type IV

Restriction endonucleases can recognize___________

A

Tandem repeats

B

Pallindromic sequences

C

GATC

D

No sequence specificity

What digestion pattern of the restriction endonucleases is followed while constructing recombinant molecule using a cosmid?

A

Partial digestion

B

Complete digestion

C

Single endonuclease digestion

D

Double endonuclease digestion

Restriction endonucleases which recognize and cut same recognition sequences are known as

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