Recombinant DNA Technology MCQ

Test your knowledge with important Recombinant DNA Technology MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Recombinant DNA Technology MCQs at Bissoy

• In baculovirus expression vector, foreign genes are expressed from the promoter of
  ক

  Polyhedrin gene

  খ

  Bacteriophage T7 gene

  গ

  E. coli lacZ gene

  ঘ

  Yeast phosphoglycerate kinase gene
• Yeast artificial chromosomes (YAC's) are used for cloning
  ক

  Large segments of DNA

  খ

  mRNA

  গ

  Bacterial DNA

  ঘ

  Yeast DNA
• Which one of the following features is not required in a prokaryotic expression vector?
  ক

  oriC

  খ

  Selection marker

  গ

  CMV promoter

  ঘ

  Ribosome binding site
• You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because
  ক

  There are no introns in the genome

  খ

  The introns contain the recognition sites and have already been spliced out

  গ

  All of restriction fragments are too small to detect

  ঘ

  Restriction endonucleases do not cut RNA, and this virus has an RNA genome
• Consider the following statements.<br>I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.<br>II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.
  ক

  Only I is true

  খ

  Both I and II are true

  গ

  Only II is true

  ঘ

  I is true and II is false
• "A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.<br>Plasmid + HindIII → 1200bp and 3800bp<br>Plasmid + BamHI → 5000bp<br>Plasmid + EcoRI → 2500bp<br>The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
  ক

  EcoRI-2; BamHI-1; HindIII-2

  খ

  EcoRI-1; BamHI-1; HindIII-2

  গ

  EcoRI-3; BamHI-2; HindIII-1

  ঘ

  EcoRI-2; BamHI-2; HindIII-1
• A plasmid cloning vector should contain all of the following sequences except
  ক

  Origin of replication

  খ

  Inducible promoter

  গ

  Selectable marker gene

  ঘ

  Multiple cloning sites
• Which of the following processes require energy?
  ক

  Ligation

  খ

  Transformation

  গ

  Restriction digestion

  ঘ

  Hybridization
• Human genome sequencing project involved the construction of genomic library in
  ক

  Bacterial artificial chromosome

  খ

  pBR322

  গ

  Bacteriophage

  ঘ

  pcDNA
• In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to<br>P. Crown gall disease in plants.<br>Q. Hairy root disease in plants.<br>R. Transfer of T-DNA into the plant chromosome.<br>S. Transfer of Ri-plasmid into the plant cell.
  ক

  S only

  খ

  P and R only

  গ

  Q and S only

  ঘ

  Q only
• A vector derived from which one of the following viruses is used for high-frequency genomic integration of a transgene in animal cells?
  ক

  Adenovirus

  খ

  Adeno-associated virus

  গ

  Lentivirus

  ঘ

  Herpes simplex virus
• Match the items in Group I with their functions in Group II.<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Group I</strong></td> <td><strong>Group II</strong></td> </tr> <tr> <td>P. rol genes</td> <td>1. Food and energy source</td> </tr> <tr> <td>Q. Opines</td> <td>2. Tumor formation</td> </tr> <tr> <td>R. Virulence genes</td> <td>3. Hairy root induction</td> </tr> <tr> <td>S. aux and cyt genes</td> <td>4. T-DNA transfer and integration</td> </tr></table> P-4, Q-3, R-2, S-1

  খ

  P-3, Q-2, R-4, S-1

  গ

  P-1, Q-3, R-4, S-2

  ঘ

  P-3, Q-1, R-4, S-2
• Hairy roots' induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by<br>P. A high degree of lateral branching.<br>Q. Genetic instability of culture.<br>R. An absence of geotropism.<br>S. Poor biomass production.
  ক

  P and R only

  খ

  P and Q only

  গ

  Q and R only

  ঘ

  R and S only
• The substrate for restriction enzyme is
  ক

  Single stranded RNA

  খ

  Partially double stranded RNA

  গ

  Cell wall proteins

  ঘ

  Double stranded DNA
• The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be
  ক

  Once every 24 bases

  খ

  Once every 256 bases

  গ

  Once every 1024 bases

  ঘ

  Once every 4096 bases
• Which one of the following is not a protease?
  ক

  Proteosome

  খ

  Trypsin

  গ

  Chrymotrypsin

  ঘ

  Peptidyl tRNA hydrolase
• DNA synthesized from an RNA template is called
  ক

  Recombinant DNA

  খ

  Transcript

  গ

  T-DNA

  ঘ

  Complementary DNA
• The restriction endonuclease HaeIII recognizes the sequence GG^↓CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice
  ক

  SmaII (CC^↓GGG)

  খ

  NotI (GC^↓GGCCGC)

  গ

  SalmIII (GG^↓CC)

  ঘ

  PstI (CTGCA^↓G)
• The enzymes that can be used in 5' end labeling of DNA are
  ক

  Alkaline phosphatase

  খ

  DNA ligase

  গ

  Terminal transferase

  ঘ

  Polynucleotide kinase
• A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?
  ক

  4.50 × 10⁵

  খ

  3.50 × 10⁵

  গ

  4.20 × 10⁵

  ঘ

  4.05 × 10⁵
• A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?
  ক

  4 labeled; 6 unlabeled

  খ

  4 labeled; 4 unlabeled

  গ

  3 labeled; 5 unlabeled

  ঘ

  3 labeled; 3 unlabeled
• Select the correct combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.
  ক

  Border repeat sequences and oncogenes

  খ

  Border repeat sequences and vir genes

  গ

  Opine biosynthetic genes and vir genes

  ঘ

  Opine biosynthetic genes and oncogenes
• To be a cloning vector, a plasmid does not require
  ক

  An origin of replication

  খ

  An antibiotic resistance marker

  গ

  A restriction site

  ঘ

  To have a high copy number
• Which one of the following cannot be a recognition sequence for a type II restriction enzyme?
  ক

  GAATTC

  খ

  AGCT

  গ

  GCGGCCGC

  ঘ

  ATGCCT
• To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from
  ক

  Intestine

  খ

  Pancreas

  গ

  Pituitary

  ঘ

  Brain
• During a positive-negative selection process, transformed animal cells expressing . . . . . . . . are killed in presence of ganciclovir in the medium.
  ক

  Pyruvate kinase

  খ

  Viral thymidine kinase

  গ

  Viral serine/threonine kinase

  ঘ

  Viral tyrosine kinase
• A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of
  ক

  Non-functional promoter

  খ

  The absence of splicing machinery

  গ

  Coupled transcription and translation

  ঘ

  Codon bias
• Antibiotic resistance marker that cannot be used in a cloning vector in Gram negative bacteria is
  ক

  Streptomycin

  খ

  Ampicillin

  গ

  Vancomycin

  ঘ

  Kanamycin
• Transfer of T-DNA from Ti plasmid into plant cell is mediated by
  ক

  Mob gene

  খ

  Vir gene

  গ

  Nif gene

  ঘ

  Octoptine gene
• BAC, which can be used to clone large DNA fragments, is derived from
  ক

  ColE plasmid

  খ

  F plasmid

  গ

  2μ

  ঘ

  Mu phage