What digestion pattern of the restriction endonucleases is followed while constructing recombinant molecule using a cosmid?

What digestion pattern of the restriction endonucleases is followed while constructing recombinant molecule using a cosmid? Correct Answer Partial digestion

Partial digestion of the cosmid vector molecules is done because total digestion will lead to small sized fragments which in turn will lead to difficulty in in-vitro packaging.
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"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?
Analysis of a hexapeptide using enzymatic cleavage reveals the following result:
• Amino acid composition of the peptide is: 2R, A, V, S, Y
• Trypsin digestion yields two fragments and the compositions are: (R, A, V) and (R, S, Y)
• Chymotrypsin digestion yields two fragments and the compositions are: (A, R, V, Y) and (R, S)
• Digestion with carboxypeptidase A yields no cleavage product.
Given: Trypsin cleaves at carboxyl side of R. Chymotrypsin cleaves at carboxyl side of Y. Carboxypeptidase A cleaves at amino side of the C-terminal amino acid (except R and K) of the peptide.
The correct amino acid sequence of the peptide is:
Assertion (A): Scientists can cut apart and paste together DNA molecules at will, regardless of the source of the molecules.
Reason (R): DNA fragments can be manipulated using restriction endonucleases and DNA ligases.
Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately
Select a figure from amongst the four alternatives, which when placed in the blank space of figure (X) would complete the pattern.
Completion of Incomplete Pattern mcq question image
Select a figure from amongst the four alternatives, which when placed in the blank space of figure (X) would complete the pattern.
Completion of Incomplete Pattern mcq question image
Select a figure from amongst the four alternatives, which when placed in the blank space of figure (X) would complete the pattern.
Completion of Incomplete Pattern mcq question image
Select a figure from amongst the four alternatives, which when placed in the blank space of figure (X) would complete the pattern.
Completion of Incomplete Pattern mcq question image
Select a figure from amongst the four alternatives, which when placed in the blank space of figure (X) would complete the pattern.
Completion of Incomplete Pattern mcq question image