The dissociation constant Kd for ligand binding to the receptor is 10-7 M. The concentration of ligand required for occupying 10% of receptors is

Receptor R is over expressed in CHO cells and analyzed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm. the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)

A

10⁴

B

10⁵

C

10⁶

D

10⁷

What property of the receptor tyrosine kinases has the ability to fine-tune the specificity of receptors for ligand binding and downstream signaling?

A

Heterodimer formation

B

Homodimer formation

C

Polymer formation

D

Monomer formation

Which subunit of the pro-receptors of tyrosine kinase receptor contains the ligand binding site?

A

Delta subunit

B

Gamma subunit

C

Alpha subunit

D

Beta subunit

The concentration of oxygen at the inner surface of the vessel is maintained at a level C depending on the pressure in the vessel, while the concentration at the outer surface is reduced to zero by the escape of oxygen to the surroundings. A steady state will eventually be reached when the concentration everywhere reaches a constant value. Provided D is independent of concentration there will be a single concentration gradient in the wall and the thickness of the wall is L, the flux is given by________________

A

DC/L

B

DL/C

C

CL/D

D

DC/L + K (some constant)

In Multiple conformation rigid-body method, a ligand is assumed to be able to adopt a number (N) of different _______ that are computed _____ the ligand being docked into the receptor.

A

low-energy conformations, after

B

low-energy conformations, prior to

C

high-energy conformations, prior to

D

high-energy conformations, after

Which among the following is an example of a case where a non-related ligand activates the receptor other than the physiological ligand?

A

Activation of TSH by antireceptor antibodies

B

Activation of TSH by Vitamin D

C

Activation of TSH by Vitamin A

D

Activation of TSH by cAMP

Receptors other than nuclear receptor superfamily have been designated as orphan receptors.

A

True

B

False

Where the thyroid hormone receptor associated protein (TRAP) complex and the vitamin D receptor interacting protein (DRIP) complex link nuclear receptors to?

A

X factor

B

General Transcription Factors (GTFs)

C

HRE

D

mRNA

In case of Protein-ligand docking, ______ ligands are often _____ in adapting their shape to fit the receptor binding pocket.

A

small molecule, highly flexible

B

large molecule, highly flexible

C

large molecule, more flexible

D

small molecule, less flexible

Which among the following tyrosine kinase receptor exist as a dimer even without the binding of ligand?

A

Epidermal Growth Factor Receptors (EGFR)

B

Insulin Receptor

C

Platelet Derived Growth Factor Receptor (PDGFR)

D

Vascular Endothelial Growth Factor Receptor (VEGFR)

The dissociation constant K_d for ligand binding to the receptor is 10^-7 M. The concentration of ligand required for occupying 10% of receptors is

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