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Which of the following is the most important Fluorescent Protein used in Flow Cytometry?

Which of the following is the most important Fluorescent Protein used in Flow Cytometry? Correct Answer GFP

There are a number of fluorescent molecules that play a very important role in Flow cytometry. These molecules absorb as well as emit the light in optimal levels. The fluorescent dyes are categorised in three major types namely Single Dyes, Tandem Dyes and Fluorescent Proteins. GFP (green fluorescent protein) is the most important fluorescent protein for understanding the protein expression of the cells. It is also considered to be the principal tool as it is used in various cases. Other fluorescent proteins include McCherry and YFP (yellow fluorescent protein). On the other hand, FITC, PE, APC are all the types of Single Dyes. These dyes use great photostability and emit bright fluorescence. 

Related Questions

Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
Directions : In each of the following questions a statement is given, followed by two conclusions. Give answer :

Statements : About 50 per cent of the animal by-products - hair, skin, horns etc. is edible protein. American chemists have developed a method of isolating 45 per cent of this protein. They used an enzyme developed in Japan to break down soya protein.

Conclusions :
I. Americans have not been able to develop enzymes.
II. Animal by-products protein has the same composition as soya protein.
Which of the following components of the X-ray fluorescent spectrometer induces fluorescent radiation?
You generate a fluorescent probe against a gene that has been deleted. You expose the DNA to the probe and observe it under fluorescent microscope. What will you see?
What are fluorescent molecules and fluorescent dyes also termed as?
Assertion (A): Light Emitting Diode (LED) lamps offer longer service life as compared to Compact Fluorescent Lamps (CFL).
Reason (R): Light Emitting Diodes (LED) lamps are more energy efficient as compared to the Compact Fluorescent Lamps (CFL).
Select the correct answer:
Flow cytometry is used to for which cell population?
The bioluminescent jellyfish Aequorea victoria, has a protein called Green Fluorescent Protein (GFP) that fluoresces with a peak wavelength of ________, under UV or blue light radiation.
Which of the following activities should be shown separately in the cash flow statement prepared as per Accounting Standard-3?
1. Cash flow from borrowing activities
2. Cash flow from operating activities
3. Cash flow from financing activities
4. Cash flow from investing activities
5. Cash flow from miscellaneous activitie
Which of the following activities should be shown separately in the cash flow statement prepared as per accounting standard-3?
I. Cash flow from borrowing activities.
II. Cash flow from operating activities.
III. Cash flow from financing activities.
IV. Cash flow from investing activities.
V. Cash flow from miscellaneous activities.