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Primary type of IF (immunofluorescence) technique uses two antibodies in association with fluorescent dye.

Primary type of IF (immunofluorescence) technique uses two antibodies in association with fluorescent dye. Correct Answer False

Primary Immunofluorescence uses only one antibody with the help of fluorescent dye. In this technique antibody interacts with the antigen. This interaction is initiated by the help of antibody which has a higher affinity to antigen. It targets the antigen and binds to it. This requires a fluorescent dye throughout so that the interaction between antibody and antigen can be observed under a microscope. Primary immunofluorescence is a faster technique as compared to secondary immunofluorescence.  

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