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Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________
The accompanying image shows a large cube made of dark and white small sugar cubes. Small cubes of the diagonally arranged row of the bottom surface of the large cube, small cubes of the row with one face on the right-hand surface of the large cube, small cubes of the row with one face on the front surface of the large cube, and small cubes of the column with one face on the top of large cube surface are all dark. Select the number of small white cubes of the large cube.
Which of the following statements are correct? 1. The DNA Technology (Use and Application) Regulation Bill, 2019 provides for the regulation of use of DNA technology for establishing the identity of certain persons. 2. DNA is made up of 26 pairs of chromosomes and provides instructions for building an entire organism and proteins. 3. DNA is composed of two chains, which coil around each other to form a double helix carrying the genetic instructions used in the growth. 4. Under the Bill, DNA profiling is allowed only in respect of criminal matters. Select the correct answer using the code given below:
Which of the following statements about DNA is/are correct? 1) DNA is the hereditary material of all living organisms. 2) All segments of DNA code for the synthesis of proteins. 3) Nuclear DNA is double helical with two nucleotide chains that run anti-parallel. 4) DNA is also found in mitochondria. Select the correct answer using the code given below:
Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because
Which of the following statements about DNA is/are correct?
1. DNA is the hereditary material of all living organisms.
2. All segments of DNA code for synthesis of proteins.
3. Nuclear DNA is double helical with two nucleotide chains which run anti-parallel.
4. DNA is also found in mitochondria.
Select the correct answer:
Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is
If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification?