Related Questions

Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
Directions : In each of the following questions a statement is given, followed by two conclusions. Give answer :

Statements : About 50 per cent of the animal by-products - hair, skin, horns etc. is edible protein. American chemists have developed a method of isolating 45 per cent of this protein. They used an enzyme developed in Japan to break down soya protein.

Conclusions :
I. Americans have not been able to develop enzymes.
II. Animal by-products protein has the same composition as soya protein.
Protein A will fold into its native state only when protein B is also present in the solution. However, protein B can fold itself into native confirmation without the presence of protein A. Which of the following is true?
Protein’s solubility depends on the relative balance between protein-solvent and protein-protein interactions.
For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.
An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.
In protein/domain analysis, each protein in the predicted proteome is again used as a query of a curated protein sequence database such as ____ in order to locate similar domains and sequences. To find orthologs, very low E value scores (E<10<20) for the alignment score and an alignment that includes 60–80% of the query sequence are generally required in order to avoid matches to paralogs.
Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).
A signaling cascade is illustrated by protein kinase protein kinase protein kinase and MAP. What purpose does this cascade serve?
Consider the following statements Enzymes and proteins can be correlated with each other in the following ways.
1. All proteins are enzyme.
2. All enzymes are protein.
3. All enzymes are not protein.
4. All proteins are not enzyme.
Which of the statements given above is/are correct?