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Doctors Medicines MCQs Q&A

Insertion of DNA fragment between PstI sites ____________ the resistance determinant.

Insertion of DNA fragment between PstI sites ____________ the resistance determinant. Correct Answer Inactivates

Insertion of DNA fragment between the PstI sites inactivates both the antibiotic resistance determinants. Hence a new selectable marker has to be used.

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Let A be an m × n matrix and Ban n × m matrix. It is given that determinant ($$I$$m + AB) = determinant ($$I$$n + BA), where $$I$$k is the k × k identity matrix. Using the above property, the determinant of the matrix given below is
\[\left[ {\begin{array}{*{20}{c}} 2&1&1&1 \\ 1&2&1&1 \\ 1&1&2&1 \\ 1&1&1&2 \end{array}} \right
Which one of the combinations of the following statements is true about antibody structure?
P. Limited proteolysis of rabbit IgG with the enzyme pepsin generates two antigen-binding regions (Fab) and an Fc fragment.
Q. Limited proteolysis of rabbit IgG with the enzyme papain generates a single bivalent antigen-binding region F(ab')2 and peptide fragments.
R. The Fc fragment of IgG can self-associate and crystallize into a lattice.
S. The F(ab')2 fragment of IgG is composed of both light and heavy chains.
The question below consists of a set of labelled sentences. These sentences, when properly sequenced form a coherent paragraph. Select the most logical order of sentences from among the options. P: Young boys and girls seem to have become inordinately addicted to these sites. Q: The parents, teachers and the government must look into the problems being caused by these networking sites and must formulate a strategy either to curb or completely stop its fast growing negative influence on the minds of our youth. R: Smart-phones, computers, social media, internet and networking sites such as Facebook and Twitter have become source of major distractions for students. S: They are thoughtlessly and uselessly wasting their precious time on these sites.
Consider the following statements :  a. As of July 2018, a total of 1092 World Heritage sites exist across 167 countries. b. Italy, with 54 World Heritage sites has the most of any country (up to July 2018). c. India has 39 World Heritage sites (up to July 2018). d. A collection of Victorian, Gothic, and Art Deco buildings of Mumbai are declared as World Heritage sites in 2018.  Which of the above statements are correct ?
The question below consists of a set of labelled sentences. These sentences, when properly sequenced form a coherent paragraph. Select the mostlogical order of sentences from among the options. P: Young boys and girls seem to have become inordinately addicted to these sites.
Q: The parents, teachers and the government must look into the problems being caused by these networking sites and must formulate a strategy either to curb or completely stop its fast growing negative influence on the minds of our youth.
R: Smart-phones, computers, social media, internet and networking sites such as Facebook and Twitter have become source of major distractions for students.
S: They are thoughtlessly and uselessly wasting their precious time on these sites.
How many PstI sites in plasmid RSF1010?
EcoRI restriction sites on a 10kb DNA fragment are shown below
Upon partial digestion, what are the lengths (in kb) of all the possible DNA fragments obtained?
You are given a DNA fragment with a CBD lesion. Which polymerase(s) will you use to replicate this fragment without taking care of the error?
A vector (cloning vector) is a carrier DNA molecule to which the human DNA fragment is attached in DNA transfer the vectors used from smallest to largest is:-