Gene Manipulation in Gram MCQ

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• The integrative vectors for P. aeruginosa have their origin of replication derived from __________
  ক

  RP4

  খ

  PMB1

  গ

  Lambda

  ঘ

  M13
• For chromosomal integration selective ____________ has to be maintained.
  ক

  Pressure

  খ

  Temperature

  গ

  Size

  ঘ

  Host range
• Genomic DNA and plasmid DNA differ in their extent of ____________
  ক

  Temperature

  খ

  Weight

  গ

  Supercoiling

  ঘ

  Hybridization
• Luciferase is a ______________
  ক

  Promoter

  খ

  Inducer

  গ

  Reporter

  ঘ

  Suppressor
• For proper ___________ analysis of a cloned gene, it must be present on the chromosome.
  ক

  Translational

  খ

  Transcriptional

  গ

  Transversal

  ঘ

  Replicative
• The high GC content of Streptomycetes affects the frequency of restriction sites.
  ক

  True

  খ

  False
• Cloning in Streptomyces is done to analyze _____________ synthesis.
  ক

  Enzyme

  খ

  Antibiotic

  গ

  Promoter

  ঘ

  Protein
• With phage vectors, plaques can be obtained in __________
  ক

  One night

  খ

  Fortnight

  গ

  One week

  ঘ

  2-3 days
• What is the average insert size of phiC31 vector?
  ক

  4 kb

  খ

  8 kb

  গ

  10 kb

  ঘ

  12 kb
• PhiC31 is a ___________ phage of Streptomyces.
  ক

  Toxic

  খ

  Transient

  গ

  Temperate

  ঘ

  Infectious
• SLP1 and pSAM2 are examples of _______________ plasmids.
  ক

  E. coli

  খ

  Streptomyces

  গ

  Agrobacterium

  ঘ

  Yeast
• SCP is a progenitor of low-copy-number vectors.
  ক

  True

  খ

  False
• SCP2 is a ______ plasmid.
  ক

  Toxic

  খ

  Antibiotic

  গ

  Sex

  ঘ

  Integrative
• Most of the vectors used with Streptomyces are derivatives of ____________
  ক

  Bacteriophages

  খ

  B. subtilis

  গ

  E. coli

  ঘ

  Pseudomonas
• GC content in gram-positive bacteria is not more than _______ percent.
  ক

  30

  খ

  50

  গ

  70

  ঘ

  90
• Blanty’s group of mini-IncP plasmids has a maximum size of _______ kb.
  ক

  1

  খ

  3

  গ

  5

  ঘ

  7
• P-group plasmids are not widely used as vectors because of their _____________
  ক

  Big size

  খ

  Small size

  গ

  Toxicity

  ঘ

  Host range
• IncP alpha and InP beta are _________ host range vectors.
  ক

  Overlapping

  খ

  Narrow

  গ

  Broad

  ঘ

  Specific
• IncP alpha plasmids are smaller in size than IncPbeta plasmids.
  ক

  True

  খ

  False
• What is the size of IncP alpha plasmids?
  ক

  20 kb

  খ

  40 kb

  গ

  60 kb

  ঘ

  80 kb
• Which resistance is lost if DNA is inserted in EcoR1 or BstEII sites?
  ক

  Sulphonamide

  খ

  Alcohol

  গ

  Ampicillin

  ঘ

  Streptomycin
• Insertion of DNA fragment between PstI sites ____________ the resistance determinant.
  ক

  Activates

  খ

  Inactivates

  গ

  Weakens

  ঘ

  Removes
• How many unique cleavage sites are located within the antibiotic resistance determinants of plasmid RSF1010?
  ক

  0

  খ

  2

  গ

  4

  ঘ

  6
• How many PstI sites in plasmid RSF1010?
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4
• What is the length of DNA of RSF1010?
  ক

  4684 bp

  খ

  6684 bp

  গ

  7684 bp

  ঘ

  8684 bp
• RSF1010 specifies resistance to how many antimicrobial agents?
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4
• If the selectable markers are not expressed in the new host, then ____________ is necessary.
  ক

  Helper phage

  খ

  Manipulations

  গ

  Temperature control

  ঘ

  Recombination
• The vector used for cloning in non-enteric bacteria must also be able to replicate in E.coli.
  ক

  True

  খ

  False
• In Gram-negative bacteria other than E.coli, it is used as a ___________
  ক

  Hybrid vector

  খ

  Main host

  গ

  Inhibitor

  ঘ

  Intermediate host
• What is required to clone DNA in non-enteric bacteria?
  ক

  Enzymes

  খ

  PCR

  গ

  Plasmid

  ঘ

  Phage