How many unique cleavage sites are located within the antibiotic resistance determinants of plasmid RSF1010?

"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"

A

EcoRI-2; BamHI-1; HindIII-2

B

EcoRI-1; BamHI-1; HindIII-2

C

EcoRI-3; BamHI-2; HindIII-1

D

EcoRI-2; BamHI-2; HindIII-1

How many PstI sites in plasmid RSF1010?

A

1

B

2

C

3

D

4

The question below consists of a set of labelled sentences. These sentences, when properly sequenced form a coherent paragraph. Select the most logical order of sentences from among the options. P: Young boys and girls seem to have become inordinately addicted to these sites. Q: The parents, teachers and the government must look into the problems being caused by these networking sites and must formulate a strategy either to curb or completely stop its fast growing negative influence on the minds of our youth. R: Smart-phones, computers, social media, internet and networking sites such as Facebook and Twitter have become source of major distractions for students. S: They are thoughtlessly and uselessly wasting their precious time on these sites.

A

PRQS

B

RPSQ

C

PSQR

D

QRPS

Consider the following statements : a. As of July 2018, a total of 1092 World Heritage sites exist across 167 countries. b. Italy, with 54 World Heritage sites has the most of any country (up to July 2018). c. India has 39 World Heritage sites (up to July 2018). d. A collection of Victorian, Gothic, and Art Deco buildings of Mumbai are declared as World Heritage sites in 2018. Which of the above statements are correct ?

A

a, b and d

B

b, c and d

C

a, b and c

D

All of the above

The question below consists of a set of labelled sentences. These sentences, when properly sequenced form a coherent paragraph. Select the mostlogical order of sentences from among the options. P: Young boys and girls seem to have become inordinately addicted to these sites.
Q: The parents, teachers and the government must look into the problems being caused by these networking sites and must formulate a strategy either to curb or completely stop its fast growing negative influence on the minds of our youth.
R: Smart-phones, computers, social media, internet and networking sites such as Facebook and Twitter have become source of major distractions for students.
S: They are thoughtlessly and uselessly wasting their precious time on these sites.

A

PRQS

B

RPSQ

C

PSQR

D

QRPS

If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?

A

The insert is not present in any of the gene

B

The insert is present in ampicillin gene but not in chloramphenicol gene

C

The insert is present in choramphenicol gene but not in ampicillin gene

D

The insert is present between both of the genes

Once a plasmid has incorporated specific genes, such as the gene coding for the antibiotic ampicillin, into its genome, the plasmid may be cloned by

A

inserting it into a virus to generate multiple copies

B

treating it with a restriction enzyme in order to cut the molecule into small pieces

C

inserting it into a suitable bacterium in order to produce multiple copies

D

running it on a gel electrophoresis in order to determine the size of the gene of interest

Consider the following statements: 1. Antibiotic resistance is one of the biggest threats to global health, food security, and development today. 2. Antibiotic resistance occurs naturally. 3. The misuse of antibiotics in humans and animals is making antibiotics less effective. Which of the following statements given above is/are correct?

A

1 and 3 only

B

1 only

C

2 and 3 only

D

1, 2, and 3

n the following passage, some of the words have been left out. Read the passage carefully and select the correct answer for the given blank out of thefour alternatives. The rising sun has great inner ______. Not just to ______ the new morning right after the dark night, but also the new rhythms and vibrations within existence. Theentire universe – including the trees, birds and flowers – wake up to the rising sun. When we listen to the birds chirping and see the flower petals open, rememberthat the sunrise is not just a great happening on the outside. If we are alert and aware, then the sunrise is also happening deep within us. Something within ourselvesis also ______ with the first rays of the sun. We just need to be sensitive and available; we just need to be ______. Like the outside world, we need to open the inner doorto let the new dawn enter within. Just open the window of our inner world and allow the beautiful experience of sunrise to ______ within ourselves – this experience isbeyond anything earthly. sunrise to ______ within ourselves

A

radiate

B

subjugate

C

accentuate

D

abdicate

Consider the following statements regarding poloyembryony and choose the correct answer:
1. False polyembryony includes the development of two or more embryo sacs within the Same nucellus, and the embryos are derived from different embryo sacs
2. In cleavage polyembryony, the zygote instead of giving rise to one embryo produces embryos by cleavage
3. According to Jeffrey (1895), in Erythronium americanum the "embryogenic mass" forms two or three embryos

A

Only 1 and 2 are true

B

Only 2 and 3 are true

C

Only 3 is true

D

1, 2 and 3 are true

How many unique cleavage sites are located within the antibiotic resistance determinants of plasmid RSF1010?

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