Bissoy.com
Doctors Medicines MCQs Q&A

Klenow fragment is the modified enzyme of which of the parent DNA polymerase?

Klenow fragment is the modified enzyme of which of the parent DNA polymerase? Correct Answer DNA polymerase I

DNA pol I attach to a short single-stranded region in a double-stranded DNA molecule and then synthesize a completely new strand, degrading the existing strand as it moves forward. Hence it possesses both nuclease and polymerase activity. Removal of the segment controlling nuclease activity renders the enzyme modified and it is then called Klenow fragment.

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Which one of the combinations of the following statements is true about antibody structure?
P. Limited proteolysis of rabbit IgG with the enzyme pepsin generates two antigen-binding regions (Fab) and an Fc fragment.
Q. Limited proteolysis of rabbit IgG with the enzyme papain generates a single bivalent antigen-binding region F(ab')2 and peptide fragments.
R. The Fc fragment of IgG can self-associate and crystallize into a lattice.
S. The F(ab')2 fragment of IgG is composed of both light and heavy chains.
In an experiment you use DNA pol I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
You are given a DNA fragment with a CBD lesion. Which polymerase(s) will you use to replicate this fragment without taking care of the error?
The Taq DNA polymerase is DNA polymerase _________ enzyme from the bacterium Thermus aquaticus.
Which of the following is false about klenow fragment?
The Klenow fragment is basically a _______________
With reference to a three-parent baby, consider the following statements: 1. The healthy nuclear DNA is removed from the patient egg cell, leaving behind faulty mitochondrial DNA. 2. Patient nuclear DNA will be transplanted to a donor’s egg with healthy mitochondrial DNA. Which of the statements given above is/are correct?
A vector (cloning vector) is a carrier DNA molecule to which the human DNA fragment is attached in DNA transfer the vectors used from smallest to largest is:-