What is the role of ethidium bromide in isopycnic centrifugation of cleared lysates?
What is the role of ethidium bromide in isopycnic centrifugation of cleared lysates? Correct Answer Intercalates and unwinds
EtBr binds by intercalating between the DNA basepairs and in doing so causes the plasmid DNA to unwind. Thus covalent circles can be separated from linear chromosomal DNA.
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Feb 20, 2025