What is the function of Dpm I endonuclease in Tm method of site directed mutagenesis?
What is the function of Dpm I endonuclease in Tm method of site directed mutagenesis? Correct Answer Sensitive to cleavage of methylated DNA
In Tm method the template DNA was deprived from an E. coli cell with an intact restriction modification system. This strand is sensitive to restriction by the Dpn I endonuclease.
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Feb 20, 2025