The second transesterification reaction occurs between __________

The second transesterification reaction occurs between __________ Correct Answer The 5’ end of exon and the 3’ splice site

The second transesterification reaction occurs between the 5’ end of exon and the 3’ splice site. In this case the newly liberated 3’-OH of the 5’ exon acts as a nucleophile. It attacks the phosphoryl group at the 3’ splice site thus joining the two exons by a phosphodiester bond.
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