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If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?
"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Once a plasmid has incorporated specific genes, such as the gene coding for the antibiotic ampicillin, into its genome, the plasmid may be cloned by
In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.
Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
Which of the following statements about gene therapy are correct?
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
A Yeast integrative plasmid cannot replicate as a plasmid inside the host?
You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?
Plates that have only ampicillin-resistant bacteria growing on them include which of the following?
Which of the following statements is/are correct? 1. Gene Editing deals with inserting genes from other organism while Gene Modification deals with altering genes that exists already within body. 2. Gene Modification process is time consuming while Gene editing is faster. Select the correct answer using the code given below.