Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments?

Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments? Correct Answer Okazaki fragments are short fragments of DNA formed on the lagging strand

Okazaki fragments are short DNA fragments on the lagging template strand during replication. They are complementary to the lagging strand.
Bissoy MCQ

Related Questions

Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease
The process of transforming the discontinuous fragments (Okazaki fragments) into a continuous molecule is referred to as
Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor
Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?
Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.
You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________
The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.
To avoid ligation of separate DNA fragments, which of the enzyme is used?
Recombinant DNA molecule is produced by ligating __________
Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is