If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

Why sticky ended ligations are carried out at temperatures lower than room temperature?

A

It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends

B

The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature

C

The enzyme carrying out ligation is unstable at low temperature

D

The sticky ends created, don’t just relegate at low temperature

In the case of blunt-end ligation, blunt ends can be generated by ___________

A

simply the action of restriction endonuclease which gives straight ends

B

the polishing of staggered ends

C

both the action of restriction endonuclease which gives straight ends and polishing of staggered ends

D

by the action of restriction endonuclease which gives staggered ends

RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

A

Blunt ended ligation

B

Addition of linkers

C

Using appropriate restriction enzymes

D

All the methods can be used equivalently

Which of the following is not a method for putting sticky ends to a blunt ended DNA fragment to be cloned?

A

Homopolymer tailing

B

Adaptors

C

Restriction digestion

D

Linkers

What is the problem associated with the use of linkers for putting sticky ends to a blunt ended molecule?

A

High ambient temperature requirements

B

Possible cleavage of the DNA molecule itself

C

High cost of synthetic linkers

D

Low compatibility of linkers

In the following passage some of the words have been left out. Read the passage carefully and select the correct answer for the given blank out of thefour alternatives. _____________ nearly eight decades, the women's movement __________ and debated the desirability and feasibility of a Uniform Civil Code,and has ended up ___________ a simple question — what is the value of uniformity? Is it for the "integrity of the nation" that uniformity in lawsis required, as some judicial pronouncements ___________? If so, who exactly is the beneficiary? Which sections of people benefit from"integrity of the nation", that abstract entity which is not exactly _______ the top of your mind as your husband throws you out on the street? and has ended up ___________ a simple question — what is the value of uniformity?

A

holding

B

positioning

C

posturing

D

posing

Consider the following statements Enzymes and proteins can be correlated with each other in the following ways.
1. All proteins are enzyme.
2. All enzymes are protein.
3. All enzymes are not protein.
4. All proteins are not enzyme.
Which of the statements given above is/are correct?

A

1 and 3

B

2 and 4

C

1, 2 and 3

D

2, 3 and 4

The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps: i) Peeling of membranes carrying away bacterial cells with it ii) Cells are lysed and denaturing of DNA is being carried out iii) Hybridization with labelled DNA iv) Placing the membrane onto plate containing recombinant cells Choose the correct sequence in which the steps are carried out (starting to ending).

A

iv)-i)-ii)-iii)

B

iv)-ii)-i)-iii)

C

i)-ii)-iii)-iv)

D

iii)-ii)-i)-iv)

Funds which combined the features of an Open-Ended and Close-Ended funds are known as which of the following?

A

Interval Funds

B

Balance Funds

C

Specialty Funds

D

None of the above

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

A

Only I is true

B

Both I and II are true

C

Only II is true

D

I is true and II is false

If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

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