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Which of the following method does not require support/matrix material?

Which of the following method does not require support/matrix material? Correct Answer Cross-linking

Cross-linking is a method of immobilization which does not require the support/matrix material. The cross-linking occurs between groups of cells/enzymes via polyfunctional reagents. Entrapment, adsorption, and covalent bonding require the use of support/matrix materials.

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