Despite the multiple coverage, sometimes certain genomic regions remain unsequenced, mainly owing to cloning difficulties.

Despite the multiple coverage, sometimes certain genomic regions remain unsequenced, mainly owing to cloning difficulties. Correct Answer True

In such mentioned cases, the remainder gap sequences can be obtained through extending sequences from regions of known genomic sequences using a more traditional PCR technique. That which requires the use of custom primers and performs genome walking in a stepwise fashion. This step of genome sequencing is also known as finishing, which is followed by computational assembly of all the sequence data into a final complete genome.
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